Japanese scientists have developed a method to isolate and identify active compounds in plant medicines. Which accurately accounts for drug behavior in the body.
Using the technique, they have identified several active compounds from Drynaria Rhizome. Traditional plant medicine, which improve memory and reduce disease characteristics in a mouse model of Alzheimer’s disease.
Traditional plant medicines used by humans for a long time. These therapies are still popular in many countries. Plants typically contain a huge variety of compounds. Many of which have no effect in the body and some which can have significant effects. If a plant medicine shows a therapeutic effect. Scientists interested isolating and identifying the compounds cause the effect.
In addition, many cases scientists repeatedly screen crude plant medicines in lab experiments. To see if any compounds show a particular effect in cells grown in a dish or in cell-free assays. If compound shows a positive effect in cells or test tubes potentially used as drug, and the scientists go on to test it in animals. However, this process is a lot of work and doesn’t account for changes that can happen to drugs when they enter the body enzymes in the blood and liver can metabolize drugs into various forms called metabolites.
However, some areas of the body, such as the brain, are difficult to access for many drugs, and only certain drugs or their metabolites will enter these tissues.
Amyloid and Tau proteins in Brain
The scientists interested finding active compounds for Alzheimer’s disease in Drynaria Rhizome, a traditional plant medicine. They used mice with a genetic mutation as a model for Alzheimer’s disease.
Meanwhile, the mutation gives the mice some characteristics of Alzheimer’s disease, including reduced memory and a buildup of specific proteins in the brain, called amyloid and tau proteins. This means that the mice are a useful tool to test potential Alzheimer’s disease treatments.
Initially, the researchers mashed the plant up and treated the mice orally using this crude plant extract. They found that the plant treatment reduced memory impairments and levels of amyloid and tau proteins in their brains.
In a key step, the team then examined the mouse brain tissue.Where treatment is needed, 5 hours after they treated the mice with the extract. Further, they found that three compounds from the plant. Made it into the brain these were a compound called naringenin and two naringenin metabolites.
Furthermore, the researchers then treated the mice with pure naringenin. Noticed the same improvements in memory deficits and reductions in amyloid and tau proteins.
Moreover, naringenin and its metabolites were likely the active compounds in the plant. They found a protein called CRMP2 that naringenin binds to in neurons, which causes them to grow, further suggesting that this could be the mechanism by which naringenin can improve Alzheimer’s disease symptoms.